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2017 | "Highly sensitive and selective electrochemical cortisol sensor using …

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Title : Highly sensitive and selective electrochemical cortisol sensor using bifunctional protein interlayer-modified graphene electrodes
Authors :Kwang Su Kim, Sung Ryeol Lim, Sung-Eun Kim, Jun Young Lee, Chan-Hwa Chung, Woo-Seok Choe, Pil J. Yoo
Journal : Sensors and Actuators B: Chemical
Vol/No/Page : 242/1121-1128
DOI : https://doi.org/10.1016/j.snb.2016.09.135

Abstract :
 Highly accurate and prompt monitoring of cortisol is imperative for the prevention of stress-related dis-eases. In this study, we present a novel electrochemical biosensor for cortisol detection harnessing anelectrochemical sensing platform consisting of a reduced graphene oxide (rGO) electrode, a bifunctionalprotein interlayer, and an antibody probe that specifically recognizes cortisol molecules. A thermallydenatured bovine serum albumin (d-BSA) protein layer was directly adsorbed as an interlayer onto therGO electrode surface via pi-stacking interactions, which provides the bifunctionality of creating covalentanchoring sites for anti-cortisol antibody probes and preventing the nonspecific binding of undefinedsubstances. Electrochemical impedance spectroscopic (EIS) measurements show that the constructedsensing platform (antibody/d-BSA/rGO) exhibits picomolar-range sensitivity cortisol detection with awide linear dynamic range spanning 10 pM–100 nM. In addition, our sensor exhibits high selectivitytoward cortisol with negligible cross-binding reactivity to the cortisol analogs, aldosterone and proges-terone. Moreover, almost complete spike recovery was confirmed with cortisol present in human salivasamples containing several interfering compounds. The demonstrated strategy for constructing the pro-tein/rGO composite sensing platform is simple but versatile. Therefore, it is expected to facilitate efficientinterfacing of various biomolecular probes with graphene-based and/or other electrode materials withhydrophobic surfaces.

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